Mindful Ramblings

Module 3 – “Kingdom Protista”

I was excited to find these wonderful websites this morning!  These will further assist you with your identification of the specimen found in your Pond Water samples.

The Microscopy-UK website is home to “The Smallest Page on the Internet”.  There is an awesome collection of microscopic image for Kingdom Monera and Kingdom Protista on both of these websites:

Microscopy – UK website 

Ron’s Pond Scum

There are also many video captures of Amoeba, Paramecium, Diatoms, etc on YouTube.com….I chose just a couple, but by doing a search for the organism you will find many videos of the species we ‘re studying:

Experiment 3.1

For some reason, I assumed that today we would be looking at mostly dead organisms!  After all, our odorous specimen have been living outside on the pool deck in the heat, trapped in a jar for almost two weeks!    There were some incredible creatures to be seen, but it took time and patience to find the good stuff.    While I was searching, a couple of verses came to mind;  One being, “Be still and know that I am God.”  (Psalm 46:10)  For if I had tried to rush through the slides to get them all done, we would have never discovered many of the following “critters”.   The other thing I thought about is the old proverb that says, “Good things come to those who wait!”

I wish you all could have been here to see this in person:

Object:  To see if other organisms could be found in the original pond samples from Module 2.  Click on images to get a larger view.

Specimen #1 – Pond Sample w/Egg

 Specimen #2 – Pond Sample with Rice

Circular specimen above may be a Diatom (Kingdom Protista, Subkingdom Algae, Phylum Chrysophyta), I think Puncticulata (species) …see thislink

Specimen #3 – Pond Sample with Rich Soil

The green, Very Hungry Caterpillar-looking, specimen in Frame A, may be a Blue-Green Algae or Cyanobacterium called Anabaena (Kingdom Monera) or a colony of Desmid (Kingom Protista, Phylum Chlorophyta).  The long, thin specimen in Frame B is a Diatom.  (There are several other diatoms in that image.)

Per Wim at Microscopy-UK, our “Big Boy” is actually a diatom, probably a Surirella species.  A very cool image of his Surirella can be found on display at theMicropolitan Museum.  You can watch its movements in this feature film I put on YouTube:

Specimen #4 – Pond Sample with Dried Grass

Frame A shows a centric diatom and the paramecium (Kingdom Protista, Subkingdom Protozoa, Phylum Ciliophora) from Frame B at 100X.   In the email from Wim, he identifies our friend in the glasses as a paramecium species known as Stylonchia.  Wait until you see his image at the Micropolitan Museum!  Incredible, right?  See if you can identify the structures we observed in Experiment 3.2, Part B.  In the video below, which is from our experiment, you can see its movement and watch the contractile vacuole doing its job!

Feel free to post your identification as a comment below, be sure to note Specimen # and Image Letter.  Who knew Biology could be so much fun!

Study Link:  Flashcards & Games for Module 3 on Quizlet